|
KMID : 0613820070170050706
|
|
Journal of Life Science
2007 Volume.17 No. 5 p.706 ~ p.713
|
|
|
Upregulation of heme oxygenase-1 by Scutellaria baica1ensis GEORGI Water-Extract in a Hypoxic Model of Cultured Rat Cortical Cells
|
|
Lee Won-Chul
Kim Wan-Sikd
Shin Gil-Cho
Moon Il-Soo
Jung Seung-Hyun
|
|
|
Abstract
|
|
|
|
Scutellaria baicalensis GEORGI (SB) is used in oriental medicine for the treatment of incipient strokes. Although it has been reported that SB is neuroprotective in a hypoxia model, its mechanism is poorly understood. Here, we investigated the effect of SB on the modulation of heme oxygenase-1 (HO-1), which has important biological roles in regulating mitochondrial heme protein turnover and in protecting against conditions such as hypoxia, neurodegenerative diseases, or sepsis. Rat cerebrocortical day In vitro(DIV)12 cells were grown in neurobasal medium. On DIV12 cells were treated with SB (20 ㎍/㎖) and given a hypoxic shock (2% O₂/5% CO₂, 3 hr) on DIV14. In situ hybridization results revealed that SB up regulated HO-1 mRNA in neuronal dendrites in both normoxia and hypoxia (38.5% and 59.2%, respectively). At the protein level, SB upregulated HO-1 in the neuronal soma in both normoxia and hypoxia (22.4% and 15.7%, respectively). Interestingly, most significant increase was associated with astrocytes, which increased HO-1 protein by 77.5% compared to SB-untreated culture. These results indicate that SB upregulates both neuronal and glial HO-1 expression, which contributes to the neuroprotection efficacy in hypoxia).
|
|
|
KEYWORD
|
|
|
rat cortical cell culture, heme oxygenase, hypoxia, Scutellaria baicalensis GEORGI
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|